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Identification of Several Clinical Isolates of Dermatophytes Based on the Nucleotide Sequence of Internal Transcribed Spacer 1 (ITS 1) in Nuclear Ribosomal DNA
Author(s) -
Mochizuki Takashi,
Kawasaki Masako,
Ishizaki Hiroshi,
Makimura Koichi
Publication year - 1999
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1999.tb03472.x
Subject(s) - internal transcribed spacer , ribosomal dna , biology , dermatophyte , spacer dna , trichophyton , restriction enzyme , nuclear dna , mitochondrial dna , ribosomal rna , dna , genetics , rapd , nucleic acid sequence , trichophyton rubrum , epidermophyton floccosum , dna sequencing , microbiology and biotechnology , gene , phylogenetics , antifungal , population , genetic diversity , demography , sociology
Nucleotide sequences of internal transcribed spacer 1 (ITS 1) in nuclear ribosomal DNA from seven morphlogically unidentified dermatophyte isolates were determined. The sequences were compared with those of typical isolates of Trichophyton (T.) mentagrophytes var. interdigitale, T. rubrum , and Epidermophyton floccosum . Two of the isolates were classified as T. rubrum and the other five as T. mentagrophytes var. interdigitale . The results did not conflict with identifications using other molecular techniques, including random amplification of polymorphic DNA (RAPD) analysis and restriction enzyme analysis of mitochondrial DNAs. Thus, the nuclotide sequence of ITS 1 is possibly a good molecular marker for identification of these major anthropophilic dermatophyte species.