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Epidermal Cytokine mRNA Expression Induced by Hapten Differs from that Induced by Primary Irritant in Human Skin Organ Culture System
Author(s) -
Matsunaga Tsuyoshi,
Katayama Ichiro,
Yokozeki Hiroo,
Nishioka Kiyoshi
Publication year - 1998
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1998.tb02428.x
Subject(s) - organ culture , hapten , cytokine , messenger rna , human skin , immunology , chemistry , medicine , biology , antibody , gene , in vitro , biochemistry , genetics
Epidermal cells produce various kinds of cytokines and express cell adhesion molecules. To analyze early events which induced in human epidermis by stimulation with various chemicals, we analyzed mRNA of cytokines expressed in epidermis in a human skin organ culture system. After painting haptens, primary irritants or vehicle control on human skin specimens sliced to 1 mm thickness and cut into approximately 5×5 mm blocks, the pieces were cultured in serum‐free medium. After separating epidermis from dermis, total RNA was extracted and mRNA of cytokines was assessed by the reverse transcriptase‐polymerase chain reaction. Only haptens induced IL‐1β mRNA at 1–3 hours. TNF‐α mRNA was induced 9 hours after application of haptens and 1 hour after application of primary irritants. IL‐1α mRNA was not induced by either haptens or primary irritants. Thus, cytokine mRNA expression induced by haptens in epidermis differs from that induced by primary irritants.