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The Proliferation and Differentiation of Neonatal Epidermal Melanocytes in F 1 Hairless Mice of HR‐1 × HR/De in Serum‐Free Culture
Author(s) -
Furuya Rikako,
Akiu Satoru,
Naganuma Masako,
Fukuda Minoru,
Hirobe Tomohisa
Publication year - 1998
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1998.tb02384.x
Subject(s) - hairless , epidermis (zoology) , endocrinology , basic fibroblast growth factor , biology , medicine , in vitro , keratinocyte , epidermal growth factor , subculture (biology) , melanocyte , fibroblast , microbiology and biotechnology , cell culture , growth factor , anatomy , biochemistry , cancer research , genetics , melanoma , receptor , botany
To investigate the characteristics of the proliferation and differentiation of epidermal melanocytes in F 1 hairless mice of HR‐1 × HR/De parents in vitro , cell suspensions of the neonatal epidermis were cultured in a serum‐free medium supplemented with dibutyryl adenosine 3‘,5‘‐cyclic monophosphate (DBcAMP) and/or basic fibroblast growth factor (bFGF). The differentiation of melanocytes was induced by treatment with DBcAMP. In contrast, the sustained proliferation of melanoblasts was induced by combined treatment with DBcAMP and bFGF. The melanoblasts could be subcultured in serum‐free medium supplemented with the two factors in the presence of keratinocytes, but not in their absence. This is the first report of successful culture of melanoblasts and melanocytes from hairless mice; it is expected to be useful in understanding the mechanism of the development of pigmented spots in the epidermis of (HR‐1 × HR/De) F 1 mice, which are reported to be induced by repeated exposure to ultraviolet light B.