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In vitro Comparison between Mouse B16 and Human Melanoma Cell Lines of the Expression of ICAM‐1 Induced by Cytokines and/or Hyperthermia
Author(s) -
Nakayama Juichiro,
Guan XiaoChun,
Nakashima Manabu,
Mashino Toshihiko,
Hori Yoshiaki
Publication year - 1997
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1997.tb02805.x
Subject(s) - melanoma , cell culture , hyperthermia , tumor necrosis factor alpha , cytokine , icam 1 , in vitro , cancer research , intercellular adhesion molecule 1 , cell , intracellular , biology , microbiology and biotechnology , immunology , cell adhesion molecule , medicine , biochemistry , genetics
The expression of intercellular adhesion molecule‐1 (ICAM‐1) in mouse B16 and human melanoma cell lines was investigated by indirect immunofluorescence using a FACScan analyzer. Mouse B16 melanoma cell lines (B16‐F1 and F10) did not express ICAM‐1 under ordinary culture conditions. Neither in vitro hyperthermia at 41°C for 3 or 6 hr nor cytokines such as γ interferon (IFN‐γ) or tumor necrosis factor α (TNF‐α) induced ICAM‐1 expression in B16 melanoma cell lines. A combination of IFN‐γ with TNF‐α caused slight induction of ICAM‐1 expression in the B16‐F10 melanoma cell line. Hyperthermia at 41°C for 3 hr in combination with the cytokines induced a slight expression of ICAM‐1 in the B16‐F1 melanoma cell line. Hyperthermia at 41°C for 3 hr or 6 hr did not induce de novo ICAM‐1 expression but hyperthermia at 43°C for 6 hr caused rather suppression of the expression of ICAM‐1 in the three human melanoma cell lines tested. In contrast, they showed a clear increase in the expression of ICAM‐1 after treatment with either with IFN‐γ or TNF‐α, and the expression was further augmented by a combination of the two cytokines. Treatment with cytokines in combination with hyperthermia at 41°C or 43°C for 3 hr did not augment the expression of ICAM‐1 over that in cytokine‐treated human melanoma cell lines, at normal temperatures. Thus, it is concluded that mouse B16‐F1 and F10 melanoma cell lines are different from human melanoma cell lines in terms of induction of ICAM‐1 expression by cytokines and/or hyperthermia.