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Secretion of Different Types of Gelatinases from Cultured Human Keratinocytes
Author(s) -
Kobayashi Takashi,
Hattori Shunji,
Nagai Yutaka,
Sakuraoka Koichi,
Nishikawa Takeji
Publication year - 1997
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1997.tb02776.x
Subject(s) - gelatinases , secretion , gelatinase , keratinocyte , gelatinase a , chemistry , microbiology and biotechnology , matrix metalloproteinase , in vitro , biology , biochemistry
We investigated gelatinolytic enzymes derived from cultured human keratinocytes. Using a zymograph, we detected 92 kDa and 72 kDa gelatinases as major components and 83 kDa as a minor component from a conditioned cultured medium (BSL‐K110, Kyokutoseiyaku Co.) of keratinocytes. After several passages, the 72 kDa band disappeared, and the 83 kDa band became dominant in another conditioned culture medium (K‐GM, Kurashikibouseki Co.); these keratinocytes had a relatively differentiated appearance. These results suggest that some mechanism may regulate the secretion of selected types of gelatinase from keratinocytes under different conditions.