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The Inhibitory Effect of Anti‐Adhesion Molecule Antibodies on Eosinophil Infiltration in Cutaneous Late Phase Response in Balb/c Mice Sensitized with Ovalbumin (OVA)
Author(s) -
Hakugawa Junichi,
Bae Sang Jae,
Tanaka Yoichi,
Katayama Ichiro
Publication year - 1997
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1997.tb02747.x
Subject(s) - ovalbumin , eosinophil , infiltration (hvac) , balb/c , antibody , chemistry , antibody response , immunology , immune system , biology , materials science , asthma , composite material
In this study, we investigated the involvement of the adhesion molecules intercellular adhesion molecule‐1 (ICAM‐1), vascular cell adhesion molecule‐1 (VCAM‐1), very late activation antigen‐4 (VLA‐4), lymphocyte function‐associated antigen 1 (LFA‐1) and macrophage antigen‐1 (Mac‐1) on eosinophil infiltration in the cutaneous late phase response (LPR) in OVA‐sensitized Balb/c mice by two approaches, immunostaining and inhibition assays with each monoclonal antibody. The eosinophil infiltration into the skin reached a peak at 12 hr after an intradermal challenge with OVA. Infiltrated eosinophils and mononuclear cells in the skin expressed Mac‐1 (eosinophils: 38.9 ± 1.55%, mononuclear cells: 51.2 ± 2.15%), LFA‐1 (eosinophils: 33.3 ± 0.95%, mononuclear cells: 23.1 ± 1.07%) and VLA‐4 (eosinophils: 14.3 ± 1.6%, mononuclear cells: 17.2 ± 1.38%) at 12 h. Intraperitoneal administration of anti‐mouse ICAM‐1, VCAM‐1, and VLA‐4 monoclonal antibodies (mAb) before the challenge decreased the eosinophil infiltration by 66.2%, 61.0%, and 54.0%, respectively. On the other hand, pretreatment with anti‐mouse LFA‐1 mAb or Mac‐1 mAb did not significantly decrease the infiltration. These results suggest that VCAM‐1/VLA‐4 interaction and ICAM‐1 play important roles in eosinophil infiltration in cutaneous LPR.