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Tissue Distribution of a Melanoma‐Associated Antigen Immunogenic in Patients with Melanoma as Analyzed by Polyclonal Antibodies to Recombinant Peptide Antigen
Author(s) -
Kako Satoru,
Hayashibe Kazuhito,
Ichihashi Masamitsu
Publication year - 1996
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1996.tb04004.x
Subject(s) - polyclonal antibodies , antigen , recombinant dna , antibody , melanoma , peptide , immunology , distribution (mathematics) , virology , medicine , biology , cancer research , gene , mathematics , biochemistry , mathematical analysis
This study aimed to detect in vivo expression of human melanoma‐associated antigen D‐1, which was identified by screening an expression cDNA library constructed from mRNA extracted from cultured melanoma cells with sera from patients with melanoma. The tissue distribution of D‐1 antigen was then analyzed. Murine anti‐D‐1 recombinant peptide polyclonal antibodies were raised by immunization of in vitro synthesized D‐1 peptide against Balb/c mice and applied immunohistochemically on paraffin‐embedded tissue specimens. D‐1 antigen was found to be restrictedly expressed on melanoma cells, but not on normal melanocytes, adjacent keratinocytes, fibroblasts, lymphocytes and adnexal structures of skin. The reactivities of anti‐D‐1 antibodies did not correlate with histogenesis of the lesions, their ability to produce melanin, and/or their primary or metastatic nature. There was no positive reactivity of anti‐D‐1 antibodies with other skin tumors, including squamous cell carcinoma, basal cell epithelioma, seborrheic keratosis, and nevus cell nevus. Further, cytoplasmic expression of D‐1 antigen in melanoma cells was observed only in a certain subgroup of patients with melanoma. This indicates that the cell surface expression of D‐1 peptide requires specific transporting proteins, such as HLA molecules.