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DNA‐Damaging Agents Induce the 72‐kD Heat Shock Protein in SV40 Transformed Normal Human Fibroblasts
Author(s) -
Muramatsu Tsutomu,
Ohno Haruhiko,
Shirai Toshihiko,
Takahashi Akihisa,
Ohnishi Takeo
Publication year - 1996
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1996.tb02659.x
Subject(s) - microbiology and biotechnology , dna damage , heat shock protein , dna , bleomycin , mitomycin c , chemistry , fibroblast , immunofluorescence , biology , biochemistry , antibody , in vitro , gene , genetics , chemotherapy
In order to elucidate the involvement of DNA damage in the induction of heat shock proteins (stress proteins), we examined the induction of 72‐kD heat shock protein (HSP72) in an SV40‐transformed human fibroblast cell line (WI38VA13) which was exposed to various DNA‐damaging agents, including 1‐(4‐amino‐2‐methyl‐5‐pyrimidinyl)methyl‐3‐(2‐chloroethyl)‐3‐nitrosourea hydrochloride, bleomycin hydrochloride, cis ‐diaminedichloroplatinum (II), mitomycin C, methylmethane sulfonate, N‐methyl‐N'‐nitro‐N‐nitrosoguanidine, and 4‐nitroquinoline‐N‐oxide. Induction of HSP72 was detected by the indirect immunofluorescence method using a monoclonal antibody. All the DNA‐damaging agents used in this study induced HSP72 on human fibroblasts. This result indicates that DNA damage is one trigger for the induction of HSP72.