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Regulatory Effects of Gamma‐Interferon on IL‐6 and IL‐8 Secretion by Cultured Human Keratinocytes and Dermal Fibroblasts
Author(s) -
Maruyama Kohji,
Zhang JianZhong,
Nihei Yoshimichi,
Ono Ichiro,
Kaneko Fumio
Publication year - 1995
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1995.tb03942.x
Subject(s) - secretion , interferon gamma , cell culture , cytokine , interleukin , biology , endocrinology , microbiology and biotechnology , medicine , chemistry , immunology , genetics
Gamma‐interferon (IFN‐ γ ) is produced by T cells and plays an important role in immunological and inflammatory processes. To determine the effects of IFN‐ γ on interleukin (IL)‐6 and IL‐8 secretion, normal human keratinocytes (NHKs), human squamous cell carcinoma cell line (HSC‐1) cells, and human dermal fibroblasts (HDFs) were incubated with 100 U/ml of recombinant (r) IFN‐ γ in the presence of various stimulants. HSC‐1 cells and HDFs spontaneously secreted both IL‐6 and IL‐8 into the culture medium. NHKs secreted detectable levels of IL‐8, but not of IL‐6, and IL‐8 secretion increased over 20 fold by stimulation with 10 nM of phorbol 12‐myristate 13‐acetate (PMA). rIFN‐ γ inhibited IL‐8 secretion in both HSC‐1 cells and PMA‐stimulated NHKs. On the other hand, it enhanced IL‐1α‐ and TNFα‐induced IL‐8 secretion in NHKs. In HDFs, rIFN‐ γ inhibited IL‐8 secretion, but enhanced secretion of IL‐6, regardless of whether they were stimulated with IL‐1 α or PMA. These results suggest that IFN‐ γ has different regulatory effects on IL‐6 and IL‐8 secretion in NHKs and HDFs, depending on the stimulus.