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Immunoglobulin Gene Analysis of Cutaneous Pseudolymphoma by Polymerase Chain Reaction
Author(s) -
Nakayama Fumiaki,
Kurosu Katsushi,
Yumoto Norio,
Mikata Atsuo
Publication year - 1995
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1995.tb03414.x
Subject(s) - pseudolymphoma , polymerase chain reaction , ethidium bromide , microbiology and biotechnology , polyclonal antibodies , antibody , immunoglobulin light chain , lymphoma , dna , pathology , biology , chemistry , gene , medicine , immunology , genetics
Polymerase chain reaction (PCR) was used to amplify the V‐D‐J region of the immunoglobulin heavy chain gene from DNA extracted from the formalin fixed, paraffin embedded skin of 3 cases of pseudolymphoma. The products were electrophoresed and observed under ultraviolet light after ethidium bromide staining. Specimens of two cases showed smears of polyclonal amplified DNA. The specimen of one case (Case 3), however, showed a single band with a smear. The presence of monoclonality in B lymphocyte populations may suggest the possibility of low grade malignancy of pseudolymphoma or transformation to malignant lymphoma in the future.