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Production and Secretion of Platelet‐Derived Growth Factor AB by Cultured Human Keratinocytes: Regulatory Effects of Phorbol 12‐Myristate 13‐Acetate, Etretinate, 1,25‐Dihydroxyvitamin D 3 , and Several Cytokines
Author(s) -
Zhang JianZhong,
Maruyama Kohji,
Ono Ichiro,
Kaneko Fumio
Publication year - 1995
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1995.tb03393.x
Subject(s) - platelet derived growth factor receptor , platelet derived growth factor , growth factor , keratinocyte , cell culture , biology , cell growth , microbiology and biotechnology , medicine , endocrinology , chemistry , cancer research , biochemistry , receptor , genetics
Platelet‐derived growth factor (PDGF) is a potent mitogen for several mesenchymal cells and plays an important role in wound repair. Three PDGF isoforms, PDGF‐AA, PDGF‐BB, and PDGF‐AB, have been found to be generated in various tissues. PDGF‐AB production by normal human keratinocytes (NHKs), by human squamous cell carcinoma cell line (HSC‐1) cells, and by human dermal fibroblasts (HDFs) was studied in the presence of agents which influence cell growth. Both NHKs and HSC‐1 cells spontaneously produced and secreted PDGF‐AB. NHKs grown in keratinocyte growth medium produced more PDGF‐AB than did those grown in keratinocyte basic medium. Phorbol 12‐myristate 13‐acetate inhibited PDGF‐AB production in NHKs but promoted its production in HSC‐1 cells. 1,25‐dihydroxyvitamin D 3 up‐regulated PDGF‐AB production, whereas etretinate did not. High levels of calcium in the culture medium induced little change in cellular PDGF‐AB levels. Prostaglandin E 1 slightly inhibited PDGF‐AB production, transforming growth factor β1 promoted PDGF‐AB production and interferon‐γ, interleukin‐1α, and tumor necrosis factor‐α failed to exert any influence at all. Cultured HDFs did not produce any detectable PDGF‐AB. These results suggest that keratinocytes are a major source of cutaneous PDGF and that this factor may therefore play an important role in wound repair and in certain proliferative skin diseases.