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Stoichiometry of the Reaction Catalyzed by Skin Sulfhydryl Oxidase
Author(s) -
Yamada Hiromichi,
Suga Yasushi,
Takamori Kenji,
Ogawa Hideoki
Publication year - 1994
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1994.tb01761.x
Subject(s) - dithiothreitol , stoichiometry , chemistry , substrate (aquarium) , catalysis , hydrogen peroxide , oxygen , enzyme , molar ratio , oxidase test , biochemistry , organic chemistry , oceanography , geology
The stoichiometry of the reaction catalyzed by skin sulfhydryl oxidase was investigated. Dithiothreitol (DTT) was used as the substrate for skin sulfhydryl oxidase. The consumption of DTT, consumption of oxygen, and production of hydrogen peroxide were measured during the enzyme reaction. The molar ratio of DTT:O 2 :H 2 O 2 in the enzyme reaction was 1:1.02:0.89. Correspondingly, the stoichiometry of the enzyme reaction was calculated to be R(SH) 2 + O 2 → + H 2 O 2 .