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Intercellular Adhesion Molecule‐1 on Cultured Human Melanoma Cells: Influence of Cytokines
Author(s) -
Yoneda Kazufumi,
Mori Shunji,
Takemura Masao,
Noma Akio,
Yamamoto Akifumi
Publication year - 1993
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1993.tb03849.x
Subject(s) - intercellular adhesion molecule 1 , cell adhesion molecule , icam 1 , intercellular adhesion molecule , lymphocyte , immune system , microbiology and biotechnology , lymphocyte function associated antigen 1 , antibody , biology , immunology , intracellular , adhesion , cell adhesion , chemistry , organic chemistry
Adhesion molecules such as intercellular adhesion molecule‐1 (ICAM‐1) and its counter‐receptor, lymphocyte function associated antigen‐1 (LFA‐1), play very important roles in immune responses. In this study, the effects of cytokines on cultured human melanoma cells (MMG2) were examined, especially focussing on the expression of ICAM‐1 on MMG2 and lymphocyte adhesion to MMG2. Both the expression of ICAM‐1 and HLA‐DR on MMG2 increased after treatment with IFN‐gamma. ICAM‐1 expression began to increase earlier than HLA‐DR expression. TNF‐alpha and IL‐1 beta also increased the expression of ICAM‐1 on MMG2. However, these cytokines did not increase the expression of HLA‐DR. IFN‐gamma had a dose dependent effect on lymphocyte adhesion to MMG2. Pretreatment of IFN‐gamma treated MMG2 with 84H10 (anti‐ICAM‐1 antibody) or pretreatment of lymphocytes with either SPV‐L7 (anti‐LFA‐1 alpha antibody) or IOT10 (anti‐LFA‐1 beta antibody) inhibited the lymphocyte adhesion to MMG2. These results suggest that ICAM‐1 molecules induced on melanoma cells by IFN‐gamma can interact with LFA‐1 molecules on lymphocytes.