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Effects of Ultraviolet‐B and PUVA on Ornithine Decarboxylase Activity, DNA Synthesis, and Protein Kinase C Activity in Mouse Skin
Author(s) -
Mizuno Nobuyuki,
Kono Takeshi,
Taniguchi Shoji,
Fukuda Michio,
Maekawa Naoki,
Hisa Tomoyuki,
Otani Shuzo,
Hamada Toshio
Publication year - 1993
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1993.tb03834.x
Subject(s) - hairless , ornithine decarboxylase , ultraviolet light , protein kinase c , protein kinase a , microbiology and biotechnology , biochemistry , dna synthesis , phorbol ester , dna , chemistry , biology , kinase , enzyme , photochemistry
Ultraviolet‐B and PUVA share several biological events with phorbol ester tumor promoters. The effects of ultraviolet‐B irradiation and topical PUVA treatment on ornithine decarboxylase activity, DNA synthesis, and protein kinase C activity, which are known to be induced or activated by phorbol ester tumor promoter, were investigated in hairless mouse skin. Ornithine decarboxylase activity was remarkably enhanced by ultraviolet‐B and PUVA. Although PUVA did not affect DNA synthesis significantly, ultraviolet‐B stimulated epidermal DNA synthesis approximately 5‐fold over control values at 48 h. However, unexpectedly, neither cytosolic nor membrane‐bound protein kinase C activity showed any change during the 2 h after either treatment. These results suggest that the protein kinase C system is not involved in the initial signal transduction system of ultraviolet‐B or PUVA, unlike the case with phorbol ester tumor promoter.