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Organ Culture of Mouse Vibrissal Hair Follicles in Serum‐free Medium
Author(s) -
Jindo Toshimasa,
Imai Ryusuke,
Takamori Kenji,
Ogawa Hideoki
Publication year - 1993
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1993.tb01379.x
Subject(s) - hair follicle , organ culture , biology , microbiology and biotechnology , andrology , medicine , genetics , in vitro
We developed a method for organ culture of mouse vibrissal hair follicles in a serum‐free medium. Cultures conducted at 31°C in 95% O 2 ‐5% CO 2 were found to be suitable for the follicles, with several findings of considerable interest pertaining to hair growth. During the 96 h culture period, the length of the isolated follicles significantly increased; the hair bulb cells maintained their normal morphology; and DNA and protein synthesis within the bulb increased time‐dependently. Furthermore, autoradiography showed that 3 H‐thymidine‐labeling was localized in the matrix cells below Auber's critical line in the hair bulb; 3 H‐leucine‐labeling was found in the epithelial region; and 35 S‐cysteine‐labeling was detected in the cortex of hair, particularly in the keratogenous zone. These results indicate that the culture system using mouse vibrissal hair would be potentially useful as an effective model for examination of hair growth.