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Dispersed Cell Culture of Human Sweat Duct Cells under Serum‐free Conditions
Author(s) -
Uchida Naoyuki,
Oura Hajimu,
Nakanishi Hideki,
Urano Yoshio,
Arase Seiji
Publication year - 1993
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1993.tb01364.x
Subject(s) - dispase , staining , sweat gland , biology , cell culture , trypsin , eccrine sweat gland , carcinoembryonic antigen , microbiology and biotechnology , pathology , anatomy , chemistry , collagenase , sweat , medicine , paleontology , biochemistry , genetics , cancer , enzyme
Human eccrine gland duct cells were successfully cultured using a serum‐free medium, K‐GM medium. Eccrine sweat ducts were isolated from dispase treated skin specimens from palms or soles. After treatment of the isolated ducts with trypsin and EDTA, dispersed cells were cultured in K‐GM medium. In primary cultures, small colonies were seen 3 to 4 days after inoculation. Then the cells rapidly proliferated and formed large colonies with a paving stone‐like cell arrangement. During the culture, small dome shaped areas were sometimes formed in the centers of colonies. Cultures multiplied for a maximum of 7 passages. The plating efficiencies of the 1st to 6th passage cells were about 20% to 30%. Immunocytochemically, cultured cells were positively stained with anti‐carcinoembryonic antigens, K8.37 and K8.13, but not with anti‐S100 protein, anti‐HLA‐DR, 34 β B4, or PKK3. An electron micrograph of the cultured cells showed a multilayer of flattened cells linked by desmosomes. These results indicate that the cultured cells possessed the staining properties compatible with those of the ductal portion of eccrine sweat glands. No contamination by other mesenchymal cells, such as fibroblasts, was seen during the culture.