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Metastatic Behavior and Tumorigenicity of a Human Melanoma Cell Line (MM‐RU) after Injection into Nude Mice
Author(s) -
Stender IdaMarie,
Etoh Takafumi,
Tsuchida Tetsuya,
Nakagawa Hidemi,
Byers H. Randolph,
Imokawa Genji,
Ishibashi Yasumasa
Publication year - 1993
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1993.tb01349.x
Subject(s) - nude mouse , cell culture , cancer research , melanoma , line (geometry) , medicine , biology , geometry , mathematics , genetics
The ability of the human amelanotic melanoma cell line MM‐RU to produce experimental metastases and to grow tumors at subcutaneous inoculation sites in 4‐week‐old nude mice was examined. After i.v. inoculation of 10 6 cells, all injected mice (n=21) developed consistent numbers of metastatic pulmonary colonies within 32 days. The coefficients of variation for the number of colonies were between 17%–23% in three independent experiments. Survival time after i.v. inoculation was 63 ± 7 days (mean ± SD) (n=20). Within 20 days, subcutaneous inoculation of 5 × 10 6 cells resulted in tumor growths of 13 ± 3 mm (mean ± SD) at the inoculation sites in all nude mice (n=12). The MM‐RU cell line seems to be a simple, fast vehicle for testing the effect of melanoma growth modulators on experimental pulmonary metastases as well as on subcutaneously growing melanoma.