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1 α ,25‐Dihydroxy Vitamin D 3 Modulation of HLA‐DR mRNA Induced by Gamma‐Interferon in Cultured Epithelial Tumor Cell Lines
Author(s) -
Tone Takeshi,
Eto Hikaru,
Katou Takako,
Otani Fumio,
Nishiyama Shigeo
Publication year - 1993
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1993.tb01343.x
Subject(s) - messenger rna , northern blot , interferon gamma , microbiology and biotechnology , cell culture , vitamin , human leukocyte antigen , biology , chemistry , endocrinology , immunology , gene , cytokine , antigen , biochemistry , genetics
Using three cultured epithelial tumor cell lines, we investigated and analyzed the effects of gamma‐interferon ( γ ‐IFN) and 1 α ,25‐dihydroxy vitamin D 3 (1,25‐(OH) 2 D 3 ) on the levels of HLA‐DR ( α ) mRNA and HLA‐DR ( β ) mRNA by Northern blot analysis. After treatment with γ ‐IFN alone, the levels of the mRNA increased. Treatment with both γ ‐IFN and 1,25‐(OH) 2 D 3 at the same time resulted in a significant decrease in the levels of mRNA in K‐TL‐1, IK‐TL‐2, and M‐TL cells as compared to those induced by γ ‐IFN alone.