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In vitro Keratin Expression of Hair Cells
Author(s) -
Kitano Yukio,
Saito Katsuyuki,
Okamoto Eriko,
Okano Yuri,
TanigakiObaoriko,
Ito Kaoru,
Tazawa Toshio,
Ito Masaaki
Publication year - 1992
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1992.tb03783.x
Subject(s) - dispase , keratin , collagenase , in vitro , trypsin , trypsinization , cell culture , microbiology and biotechnology , chemistry , scalp , dandruff , biology , biochemistry , shampoo , enzyme , anatomy , paleontology , genetics , organic chemistry
Abstract Human hair follicles were isolated from the scalp by dispase and collagenase treatment and dispersed into a cell suspension by trypsin. These cells proliferated well and could be subcultured 7 to 8 times. The medium used was MCDB 153 HAA medium further supplemented with some amino acids, hydrocortisone, insulin, EGF, and bovine brain extract. The concentration of Ca ++ was adjusted to 0.1 mM. Immunohistochemically, these cells were proved to possess keratins specific to hair forming cells.