Premium
The ATPase‐staining of Langerhans Cells in Previously Stored Skin Tissues
Author(s) -
Kim YoungKeum,
Miyauchi Hiroko,
Horio Takeshi,
Asada Yasuo
Publication year - 1991
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1991.tb03109.x
Subject(s) - staining , stain , vital stain , atpase , periodic acid–schiff stain , pathology , guinea pig , chemistry , biology , biochemistry , enzyme , medicine , endocrinology
The ATPase‐staining of Langerhans cells is usually done on fresh, unfixed biopsy specimens. In the present study, we attempted to stain these cells in previously stored tissues of guinea pig skin. The ATPase activity disappeared after freezing or preservation in formalin or ethanol. In contrast, tissue specimens which had been stored in physiological saline at 4°C stained as well as fresh cells even after 14 days.