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Functional Analysis of HLA‐DR‐expressing Keratinocytes from Tuberculin Reactive Skin
Author(s) -
Morikawa Mitsuya,
Yasue Takashi,
Ohashi Masaru
Publication year - 1991
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1991.tb03066.x
Subject(s) - keratinocyte , antigen , immunology , monoclonal antibody , peripheral blood mononuclear cell , in vivo , tuberculin , in vitro , chemistry , biology , microbiology and biotechnology , antibody , medicine , pathology , tuberculosis , biochemistry
We examined the functional roles of HLA‐DR + keratinocytes which were induced in vivo from tuberculin reactive skin. At 4 days after intradermal PPD injection, about 80% of keratinocytes obtained from the tuberculin reactive area expressed DR antigens. In 14 of 18 individuals examined, PPD‐pulsed DR + keratinocyte fraction induced autologous T‐cell proliferation. The proliferative response was PPD‐dependent, antigen specific; it depended upon DR expression by the keratinocyte fraction, because it did not occur in the presence of the PPD‐nonpulsed DR + keratinocyte fraction and was completely blocked by mouse monoclonal antibody to HLA‐DR. However, the antigen‐presenting capacity of the DR + keratinocyte fraction appeared to be less than that of monocytes or the DR + epidermal cell fraction. The DR + keratinocyte fraction was also able to stimulate allogeneic T‐cell DNA synthesis, but the DR − keratinocyte fraction could not. The possible influences of contaminant Langerhans cells and mononuclear cells in the DR + keratinocyte fraction were considered to be unlikely. These results suggest that DR + keratinocytes, which are induced in vivo , can both present the antigen to autologous T cells and stimulate allogeneic T cells.