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Unusual Fatty Acid in Sporothrix schenckii
Author(s) -
Yamada KenIchi
Publication year - 1990
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1990.tb01710.x
Subject(s) - sporothrix schenckii , sporotrichosis , fatty acid , chemistry , chromatography , fungus , gas chromatography , biology , biochemistry , botany , immunology
Sporothrix schenckii was isolated from a specimen from a 75‐year‐old woman with lymphatic sporotrichosis on the left forearm. A mass of fungi was cultured in liquid Sabouraud dextrose broth for three months. Crude lipids were extracted from the cultured fungal mass. Total fatty acids and free fatty acids were isolated, purified, and then analyzed using thin‐layer chromatography, gas liquid chromatography, and mass spectrometry. Total fatty acid composition consisted of seven molecular species of C 15:0 (2.5%), C 16:1 (2.5%), C 16:0 (64.5%), 2‐Me‐C 16:0 (5.1%), C 17:0 (2.0%), C 18:1 (18.0%) and C 18:0 (5.3%). Free fatty acid composition consisted of eight species of C 14:0 (2.0%), C 15:1 (4.1%), C 16:1 (7.5%), C 16:0 (68.5%), C 17:0 (0.6%), C 18:2 (2.0%), C 18:1 (14.5%) and of C 18:0 (0.9%). 2‐Me‐C 16:0 was detected in the fungus for the first time.