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Effects of Human Recombinant Tumor Necrosis Factor‐α (TNF‐α) on the Proliferative Potential of Human Keratinocytes Cultured in Serum‐free Medium
Author(s) -
Kono Takeshi,
Tanii Tsukasa,
Furukawa Masayoshi,
Mizuno Nobuyuki,
Taniguchi Shoji,
Ishii Masamitsu,
Hamada Toshio
Publication year - 1990
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1990.tb01667.x
Subject(s) - tumor necrosis factor alpha , recombinant dna , adhesion , cell growth , cell culture , necrosis , keratinocyte , growth factor , chemistry , tumor necrosis factor α , biology , microbiology and biotechnology , endocrinology , in vitro , medicine , biochemistry , receptor , genetics , organic chemistry , gene
The effects of human recombinant tumor necrosis factor‐α (TNF‐α) on human keratinocytes cultured in a serum‐free medium were investigated. TNF‐α markedly suppressed cell growth. The growth‐inhibitory effect was reversible and cytostatic at a concentration of 1–5 U/ml, but appeared to be irreversible and cytocidal at 10 U/ml. The growth suppressive effect was more marked when TNF‐α was added in the late growth phase or preconfluent phase than when it was added in early or mid‐growth phases. No effects of TNF‐α on cell adhesion to the substrate were observed. These results indicate that TNF‐α is a very potent anti‐proliferative agent for human keratinocytes.