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Human Squamous Cell Carcinoma from Skin: Establishment and Characterization of a New Cell Line (HSC‐5)
Author(s) -
Hozumi Yutaka,
Kondo Shigeo,
Shimoura Takako,
Aso Kazuo
Publication year - 1990
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1990.tb01615.x
Subject(s) - cell culture , in vitro , transplantation , doubling time , cell , pathology , basal cell , carcinoma , biology , human skin , microbiology and biotechnology , cancer research , medicine , biochemistry , genetics
A new cell line, designated as HSC‐5 and derived from human skin squamous cell carcinoma (SCC), has been established in vitro and maintained proliferative in continuous tissue culture for over two years. The cells grow in a monolayer in vitro and have anaplastic epithelioid features. The doubling time was about 35hr at the 30th passage. Chromosome analysis showed hypotetraploidy with a modal number of 76. A trial of transplantation of the cultured cells into nude mice was not successful. Analysis of cytokeratins from HSC‐5 by two‐dimensional gel electrophoresis revealed polypeptides No. 5, 8, 13, 18 and 19. The cell line is available to other investigators.