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A New Method for Culturing Human Hair Follicle Cells on Floating Mixed Collagen Membranes
Author(s) -
Kuwana Ryuichiro,
Arase Seiji,
Sadamoto Yasushi,
Takeda Katsuyuki
Publication year - 1990
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1990.tb01602.x
Subject(s) - membrane , hair follicle , microbiology and biotechnology , chemistry , follicle , biology , biochemistry , endocrinology
A new method for culturing human hair follicle cells without a biological feeder layer has been developed. Dispersed hair follicle cells obtained from plucked hairs have been successfully grown on floating mixed collagen membranes (collagen types I and IV) and have reached a confluent state. They could be subcultured up to twice. Differentiation of the cells was maintained throughout a month in culture. Stratification of the cells and continuous epithelial pavement formation were seen, as in cultured epidermal keratinocytes.