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A Study of Mosquito Salivary Gland Components and Their Effects on Man
Author(s) -
Oka Keiko,
Ohtaki Noriko,
Yasuhara Tadashi,
Nakajima Terumi
Publication year - 1989
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1989.tb01587.x
Subject(s) - salivary gland , aedes albopictus , histamine , chemistry , biology , medicine , chromatography , endocrinology , biochemistry , aedes aegypti , ecology , larva
To evaluate the mechanism of mosquito bite reaction in man, salivary gland extracts from female Aedes albopictus were prepared. When the extract of the gland was analysed by ion‐exchange high performance liquid chromatography the amount of histamine in a pair of salivary glands of one mosquito was found to be below the limit of detection, an amount which is insufficient to produce an immediate reaction. Salivary gland extracts were fractionated to higher and lower molecular weight components. Intradermal injection tests with salivary gland extracts, which contained less than 100 ng of protein showed that the higher molecular weight fraction (molecular weight >10,000) elicited an immediate and a delayed reaction similar to a bite reaction.