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Establishment of a Human Dermatofibrosarcoma Protuberans Cell Line: Cytological Characteristics
Author(s) -
Shindo Yasuko,
Akiyama Junichi,
Yamaji Kazuhiko,
Ishihara Yoko,
Saida Toshiaki,
Takase Yoshio
Publication year - 1989
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1989.tb01280.x
Subject(s) - dermatofibrosarcoma protuberans , histiocyte , pathology , biology , phagocytosis , acid phosphatase , population , receptor , cell culture , microbiology and biotechnology , immunology , medicine , enzyme , biochemistry , genetics , environmental health
We established a dermatofibrosarcoma protuberans (DFSP) cell line derived from a DFSP tumor from a 69‐year‐old female. The population doubling time of the cells was about 45 hours. The cells were mostly round, although a few triangular or spindle‐shaped cells were also found. They could not be transplanted into nude mice. These cells had some histiocyte characteristics which are not observed in fibroblasts: 1) Electron microscopically, there were more lysosomes and phagolysosomes than in fibroblasts. Dense bodies and myelin figures derived from lysosomes were also observed. 2) Staining with acid phosphatase (ACPase), esterase, and α‐1‐antitrypsin was positive. 3) The cells had C3 receptors but no Fc receptors. However, there were two differences between DFSP cells in the established line and those in the early stages: in the former, the capacity for immunophagocytosis (Fc receptors and phagocytosis of Candida albicans ) and collagen synthesis were almost entirely absent.