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Chemotactic Responsiveness of Eosinophils Isolated from Patients with Inflammatory Skin Diseases
Author(s) -
Morita Eishin,
Schröder JensMichael,
Christophers Enno
Publication year - 1989
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1989.tb01278.x
Subject(s) - chemotaxis , eosinophil , immunology , leukotriene b4 , medicine , inflammation , platelet activating factor , psoriasis , atopic dermatitis , granulocyte , eosinophil cationic protein , receptor , asthma
We determined the chemotactic responsiveness of peripheral eosinophilic granulocytes (eosinophils) isolated from patients with inflammatory dermatoses and healthy volunteers. Ten patients with atopic dermatitis, five patients with drug reactions, ten patients with psoriasis, and fourteen healthy volunteers were studied. Well characterized chemotaxins, the complement split product C5a, leukotriene B 4 (LTB 4 ), platelet activating factor (PAF), and N‐formyl‐methionylleucyl‐phenylalanine (FMLP), were used as chemoattractants. Eosinophils from healthy volunteers showed strong migratory responses towards C5a and PAF but responded poorly to LTB 4 and FMLP. When patients were grouped by disease severity, eosinophil chemotactic responses to PAF were significantly enhanced in severely affected patients (p<0.05); this was not true with C5a, LTB 4 or FMLP. This enhanced eosinophil chemotaxis to PAF was not related to a specific disease. No correlation between eosinophil chemotactic activity and peripheral blood eosinophil count was observed. The increased responsiveness of circulating eosinophils towards PAF may be related to altered receptor expression during cutaneous inflammation.