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Effects of 1α,25‐Dihydroxyvitamin D 3 on the Transglutaminase Activity of Transformed Mouse Epidermal Cells in Culture
Author(s) -
Lee Seung Churl,
Ikai Kouichi,
Ando Yoshihiro,
Imamura Sadao
Publication year - 1989
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1989.tb01212.x
Subject(s) - tissue transglutaminase , retinoic acid , chemistry , cell culture , tetradecanoylphorbol acetate , calcitriol , endocrinology , vitamin , phorbol , medicine , dexamethasone , prostaglandin e2 , vitamin d and neurology , microbiology and biotechnology , enzyme , biochemistry , biology , protein kinase c , genetics , gene
Induction of the transglutaminase activity of a transformed mouse epidermal cell line (PAM 212 cells) by 1α,25‐dihydroxyvitamin D 3 (1α,25‐(OH) 2 ‐D 3 ), the active form of vitamin D 3 , was investigated. Addition of 1α,25‐(OH) 2 ‐D 3 to a culture medium stimulated twice the transglutaminase activity at a concentration of 10 −7 M, but vitamin D 3 , prostaglandin E 1 , E 2 , and F 2 a failed to show this induction. Phorbol 12‐o‐tetradecanoylphorbol‐13‐acetate (TPA) and ***dexamethasone also induced an increase in transglutaminase activity. Exposure to both 1α,25‐(OH) 2 ‐D 3 and retinoic acid caused remarkably synergistic effects on the induction of transglutaminase in the PAM 212 cells. In contrast, simultaneous addition of 1α,25‐(OH) 2 ‐D 3 and TPA was antagonistic and resulted in less than additive induction. Vitamin D 3 also showed a similar but lesser effect. These results suggest that 1α,25‐(OH) 2 ‐D 3 induces the transglutaminase activity via mechanisms disparate from those of retinoic acid and modifies epidermal differentiation.