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In vitro and In vivo Regulation of Transglutaminase Activity of Mouse Epidermal Cells by Various Vitamin A Compounds
Author(s) -
Lee Seung Churl,
Ikai Kouichi,
Ando Yoshihiro,
Danno Kiichiro,
Imamura Sadao
Publication year - 1988
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1988.tb04074.x
Subject(s) - tissue transglutaminase , retinoic acid , in vivo , retinyl acetate , in vitro , chemistry , biochemistry , tretinoin , vitamin , cell culture , pharmacology , enzyme , microbiology and biotechnology , biology , genetics , gene
In vitro and in vivo effects of various vitamin A analogues on the transglutaminase activity of mouse epidermal cells were investigated. In vitro addition of all‐trans‐retionic acid (retinoic acid), retinyl acetate, or Ro 10‐9359 (etretinate) to a culture medium induced transglutaminase activity in a transformed mouse epidermal cell line (PAM 212 cells). Induction by a new synthetic vitamin‐A‐like compound (E‐5166, polyprenoic acid derivative) was significant but less marked than that by other vitamin A analogues. Simultaneous addition of retinoic acid and the phorbol ester 12‐ O ‐tetradecanoylphorbol‐13‐acetate (TPA), which also induces transglutaminase, did not cause any increase in transglutaminase activity. These retinoic acid derivatives inhibited the proliferation of PAM 212 cells at concentrations of 10 –5 –10 –6 M in the culture medium. In vivo analysis consisted of oral administration of retinoic acid and Ro 10‐9359 to adult mice. These mice were found to have increased liver transglutaminase activity in the soluble fraction but not in the particulate fraction. Orally administered retinoic acid did not induce transglutaminase activity in the epidermis in the whole skin. These results suggest that retinoids modify transglutaminase activity and epidermal proliferation and differentiation in vitro , but the induction of the enzyme seems not to be related to the clinical effects of orally administered retinoids on various skin diseases.