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Conserved Gene Organization of 67K Keratin in Patients with Hereditary Palmoplantar Keratoderma
Author(s) -
Tanaka Toshihiro,
Horiguchi Yuji,
Moriwaki Shinichi,
Danno Kiichiro,
Ikai Kouichi,
Imamura Sadao
Publication year - 1988
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1988.tb03694.x
Subject(s) - palmoplantar keratoderma , keratin , keratoderma , restriction enzyme , biology , genetics , gene , keratin 8 , microbiology and biotechnology , keratin 6a , restriction fragment length polymorphism , hyperkeratosis , intermediate filament , genotype , cytoskeleton , cell
Restriction enzyme analysis to determine the divergence of the gene organization and the restriction fragment length polymorphism in four patients with two types of hereditary palmoplantar keratoderma revealed that the gene organization of 67K keratin was conserved in all cases. No restriction enzyme fragement length polymorphism was observed in a family of the Vörner type (Epidermolytic hereditary palmoplantar keratoderma) nor in a family of the Unna‐Thost type. Total southern blotting analysis of both patients and controls showed the same pattern of bands; digestion with Bam HI revealed 16.5 Kilo base pairs (Kbp) and 8.5 Kbp, whereas double digestion with Bam HI and Eco RI showed bands of 7.2 Kbp and 4.5 Kbp, and that with Bam HI and Sph I gave 6.0 Kbp and 1.7 Kbp. These results indicate that abnormalities in the keratin pattern, especially a decrease in the amount of higher molecular weight keratin in Vörner type palmoplantar hyperkeratosis, are not due to changes in the gene organization, such as insertion and/or deletion in the 67K keratin gene, and that there is no polymorphism around the 67K keratin gene of either type.