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The Distribution of 2,4‐Dinitrophenyl Groups on Thy‐1 Positive Cells in the Epidermis of Mouse Following Skin Painting with 2,4‐Dinitrochlorobenzene
Author(s) -
Bang Dongsik,
Nakagawa Shojiro,
Oka Daisuke,
Takei Yoji,
Jinno Yoko,
Ueki Hiroaki
Publication year - 1988
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1988.tb03644.x
Subject(s) - dinitrophenyl , epidermis (zoology) , chemistry , microbiology and biotechnology , monoclonal antibody , in vivo , antibody , immunology , biology , anatomy
The distribution of 2,4‐dinitrophenyl (DNP) groups on Thy‐1 positive epidermal cells in vivo after 2,4‐dinitrochlorobenzene (DNCB) was painted on the skin surface of C3H/He mice was examined. Epidermal cell suspensions (EC) derived from trypsin‐separated mouse ear skin painted with DNCB were double‐stained for DNP groups and Thy‐1 protein using FITC‐anti‐DNP antibody, biotinylated anti‐mouse Thy‐1,2 monoclonal antibody, and phyco‐erythrin conjugated avidin. There were on the average 1.7% Thy‐1 + ECs; DNP groups were detectable on 77.8% of them when the specimen was treated with anti‐DNP followed by anti‐Thy‐1,2 incubation. The incidence of Thy‐1,2 + EC with DNP groups decreased when cells were treated with these antibodies in the reverse order. The significance of these findings is discussed in relationship to the mode of DNCB Thy‐1 + EC binding.