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Fractionation of Epidermal Keratinocytes by Counterflow Centrifugal Elutriation (CCE)
Author(s) -
Sakamoto Fumino,
Nomura Hirofumi,
Hachisuka Hiroshi,
Sasai Yoichiro,
Shiotsuki Kazuhide
Publication year - 1988
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1988.tb03642.x
Subject(s) - elutriation , percoll , fractionation , sedimentation coefficient , sedimentation , chromatography , chemistry , biophysics , centrifugation , biology , biochemistry , organic chemistry , enzyme , paleontology , sediment
Counterflow centrifugal elutriation (CCE) is one of the most powerful and rapid methods for separating free cells by sedimentation velocity based on cell size, density, or shape. To obtain a sufficient number of cells, the fractionation of guinea pig epidermal keratinocytes was developed with the use of a newly‐developed elutriation system. An EDTA‐trypsin method was used to isolate keratinocytes; they were then separated into three groups according to flow rate by CCE. Morphologically, the keratinocytes in each region were identified as basal cells, spinous cells, and granular cells. The DNA distribution pattern agreed well with that obtained by Percoll's density gradient. The light‐scatter of keratinocytes in each fraction obtained by the present method was similar to that obtained with the density gradient method.