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Effects of L‐glutamine on Tyrosinase and γ ‐glutamyl Transpeptidase of B‐16 Melanoma Cells in Culture
Author(s) -
Chakraborty Chandan,
Hatta Susumu,
Ichihashi Masamitsu,
Hayashibe Kazuhito,
Mishima Yutaka
Publication year - 1988
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1988.tb03640.x
Subject(s) - gtp' , glutamine , melanoma , tyrosinase , chemistry , enzyme , hela , biochemistry , cell culture , microbiology and biotechnology , in vitro , amino acid , biology , cancer research , genetics
Omission of L‐glutamine (Gln) from the culture medium decreases tyrosinase activity and increases γ ‐GTP activity in cultured melanotic B‐16 melanoma cells; inclusion of this amino acid in the medium shows the reverse effects. Its absence, however, decreases the γ ‐GTP activity in non‐pigmented cells like HeLa cells, fibroblasts and amelanotic melanoma cells and its presence induces a dose dependent increase in this enzyme activity in these cells. The decrease of γ ‐GTP activity in the melanotic melanoma cells by Gln is attributed to increased production of dopa and its derivatives by these cells, as evidenced by our results. Our data also indicate the inhibitory effects of dopa and several other dopa derivatives known to be produced by the melanoma cells on isolated bovine kidney γ ‐GTP.