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Negative Immunolabelling for Factor VIII‐related Antigen in the So‐called “Sclerosing Haemangiomas” (Histiocytofibromas) of the Skin
Author(s) -
Kanitakis Jean,
Hermier Claude,
Mauduit Gilles,
Thivolet Jean
Publication year - 1987
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1987.tb03587.x
Subject(s) - histiocyte , immunohistochemistry , pathology , polyclonal antibodies , antigen , antibody , avidin , cell type , chemistry , cell , biotin , biology , medicine , immunology , biochemistry
The cellular composition of the so‐called “sclerosing haemangioma” (or histiocytofibroma‐HF) of the skin has long been controversial and is still uncertain. Histiocytes, fibroblasts and endothelial cells have been considered as the cell‐type of origin of these lesions. In order to assess the alleged endothelial derivation of HF cells, we performed an immunohistochemical study of 22 cases of HF of the skin using a polyclonal antibody to factor VIII‐related antigen (F VIII RA) and an avidin‐biotin‐peroxidase technique on formalin‐fixed, paraffin‐embedded sections. F VIII RA distinctly decorated the small vessels around and inside the tumours, but HF cells themselves were negative. This result, which agrees with previous enzyme‐histochemical studies, speaks against the endothelial origin of HF cells.