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False‐positive Thormahlen Test Induced by Latamoxef Sodium
Author(s) -
Murakami Shinji,
Shiraishi Satoshi,
Miyauchi Shunji,
Miki Yoshiharu
Publication year - 1987
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1987.tb03567.x
Subject(s) - latamoxef , chemistry , chromatography , high performance liquid chromatography , urine , residue (chemistry) , cephalosporin , organic chemistry , biochemistry , antibiotics
Intravenous administration of latamoxef sodium (LMOX) induced a false‐positive Thormählen test. When LMOX‐containing urine was alkalinized, the LMOX appeared to dissociate into N‐methyltetrazolthiol (N‐MTT) and a remainder. After subsequent purification of the Thormählen‐positive fraction with methyl ethyl ketone and twice repeated preparative thin‐layer chromatographies (PTLC), a crystalline residue was obtained. It was found to be almost identical to N‐MTT in high performance liquid chromatography (HPLC) pattern and 1 H‐NMR analysis.