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Heterogeneity in Basement Membranes of Skin—Electrostatic Properties of Basal Lamina‐associated Anionic Sites
Author(s) -
Kazama Takashi
Publication year - 1987
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1987.tb03562.x
Subject(s) - cationic polymerization , capillary action , ruthenium red , basement membrane , membrane , chemistry , ruthenium , basal lamina , biophysics , anatomy , materials science , polymer chemistry , biochemistry , organic chemistry , biology , calcium , composite material , ultrastructure , catalysis
In order to evaluate the charge‐selective filter function of basement membranes (BMs), electrostatic properties of anionic sites on the BMs of dermo‐epidermal junction were compared with those of the nuclear (the thick capillary wall) and the attenuated (the thin capillary wall) portions of capillary and other small blood vessels by determining salt concentrations required to remove ruthenium red from these BMs of the rat skin previously perfused with the cationic dye. The concentration of NaCl which was required to remove ruthenium red was more than 1.4M for the attenuated portion of capillary and only 1.2M for the other BMs, indicating that the former is more highly negatively charged than the latter. It is suggested that the BM of the attenuated portion of the capillary expedites the charge‐selective exchange of macromolecules more effectively than the other BMs.