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Clonal Variation in Expression of p97 Antigen in Human Melanoma Cell Lines Detected by Mixed Passive Hemagglutination
Author(s) -
Saida Toshiaki,
Uno Akihiko
Publication year - 1987
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1987.tb03561.x
Subject(s) - antigen , biology , cell culture , monoclonal antibody , microbiology and biotechnology , hemagglutination , clone (java method) , melanoma , antigenic variation , virology , antibody , immunology , cancer research , genetics , gene
Abstract The expression of a melanoma‐associated antigen (MAA), p97, on the cell surfaces of five human melanoma cell lines was investigated with the recently developed mixed passive hemagglutination (MPHA) method. Two of these lines, HMV‐I and HMV‐II, were subclones separated by cloning from a common original cell line. In all cell lines except HMV‐I, definite expression of p97 antigen was detected in MPHA However, virtually no expression of the antigen was observed in the HMV‐I cell line. These results were confirmed by immunoelectron microscopic study. The present study is meaningful for the following two reasons. First, it was shown that MPHA is a sensitive and conventional method for detection of tumor‐associated surface antigens on cultured neoplastic cells. Second, this is the first report of clonal variation in expression of p97 antigen between subclones of a human melanoma cell line. Clonal variation in expression of a MAA as shown in the present study may cause serious problems when a monoclonal antibody is used for immunodiagnosis or immunotherapy.