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Hyperthermic Effects on Peripheral Lymphocytes Isolated from a Chronic Lymphocytic Lymphoma/Leukemia Patient In vitro
Author(s) -
Nakayama Juichiro,
Yasumoto Shinichiro,
Kabemura Mayumi,
Imayama Shuhei,
Asahi Masakazu,
Urabe Harukuni
Publication year - 1987
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1987.tb03559.x
Subject(s) - incubation , chronic lymphocytic leukemia , in vitro , hyperthermia , cytoplasm , lymphoma , organelle , leukemia , atypical lymphocyte , lymphocyte , thymidine , vacuole , pathology , biology , immunology , medicine , chemistry , microbiology and biotechnology , biochemistry
Effects of hyperthermia in vitro on the atypical lymphocytes isolated from peripheral blood of a chronic lymphocytic lymphoma/leukemia patient were investigated. Incubation of the isolated lymphocytes at 43 ° C for 5 hr killed 15% of the cells, while more than 95% of the lymphocytes isolated from normal individuals and heated under the same conditions survived. Cultivation of the patient's lymphocytes at 37° C for 4 days after heat treatment at 43 ° C for 5 hr resulted in a viability of less than 50%. Proliferative responses of the patient's lymphocytes to lectins showed 70% more inhibition than did the control when heated at 43 ° C for 1 hr and were abolished completely after 3 hr of hyperthermia. Although, in normal individuals, complete recovery of ( 3 H)‐thymidine uptake of the lymphocytes heated at 43 ° C for 3 hr was observed after 4 days of cultivation at 37 ° C, this recovery did not occur in the patient's cells. Morphological changes in the atypical lymphocytes heated at 43 ° C for 3 hr occurred in the cytoplasmic organelles; in particular, enlargement of mitochondria, increased numbers of cytoplasmic granules and vesicles, probably derived from lysosomes, and hyperproduction of myelin sheath‐like structures around the nuclear membrane were seen.