Premium
Fibrous Proteins of Trichilemma‐oid Epidermal Keratinization without Keratohyalin Granules Formation
Author(s) -
Tezuka Tadashi,
Takahashi Masae
Publication year - 1987
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1987.tb03540.x
Subject(s) - stratum corneum , keratin , stratum granulosum , chemistry , epidermis (zoology) , sweat , biology , biochemistry , microbiology and biotechnology , anatomy , genetics , paleontology
Interactions between the histidine‐rich protein of keratohyalin granules and keratin fibers were investigated. Keratin fibers of the stratum corneum from sole skins of both normal persons (NP) and those with hereditary palmoplantar keratoderma (HPPK) were examined. HPPK samples showed trichilemma‐oid keratinization except at the sweat duct. Epidermis around the sweat duct showed stratum granulosum formation. The keratin pattern was lost in the non‐sweat duct region; any changes in the size and distribution‐pattern of keratin fibers were, however, observed by electron microscopy. In sodium dodecylsufate polyacrylamide gel electrophoresis (SDS‐PAGE), keratin fibers in the HPPK stratum corneum, from both non‐sweat duct and sweat duct regions, and in the stratum corneum of the sole lesion of the HPPK's mother, lacked 57kd subunits, but the electrophoretic profiles of other keratin subunits were identical to those from NP samples. It was concluded that the lack of histidine‐rich protein formation causes no change in keratin fibers themselves either under electron microscopy or in SDS‐PAGE. The absence of a 57kd subunit keratin fiber may have been caused by abnormalities in the production or degeneration processes of keratin fibers of the HPPK epidermis.