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Low Glucose‐6‐Phosphate Dehydrogenase Activity in Cultured Skin Fibroblasts from Werner's Syndrome
Author(s) -
Shindo Yasuko,
Akiyama Junichi,
Matsumoto Kazuhiko,
Takase Yoshio,
Hashimoto Takashi
Publication year - 1986
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1986.tb02963.x
Subject(s) - dehydrogenase , glucose 6 phosphate dehydrogenase , phosphate , enzyme , cell culture , biochemistry , chemistry , biology , medicine , endocrinology , genetics
Abstract Fibroblasts from leg skin of a patient with Werner's syndrome were cultured. Their growth rate was lower than that of the normal control. They were variable in size and did not reach confluence. The activity of glucose‐6‐phosphate dehydrogenase (G6PDH) in this cell line was one seventh that of the normal control. The results of pulse labeling and chase experiments indicated that the rate of synthesis of G6PDH in the line was low, in agreement with the decrease in enzyme quantity.