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Distribution Pattern of DNA Polymerases in Epidermis
Author(s) -
Kaneko Noriaki,
Tanaka Takaho,
Hidaka Toshihiro,
Ogura Ryohei
Publication year - 1986
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1986.tb02953.x
Subject(s) - dna polymerase , epidermis (zoology) , polymerase , biology , microbiology and biotechnology , dna polymerase ii , stratum granulosum , dna clamp , dna polymerase i , dna , polymerase chain reaction , biochemistry , anatomy , genetics , stratum corneum , gene , reverse transcriptase
DNA polymerases are known to play important roles in DNA replication and repair processes. The present study revealed that the DNA polymerase α and β participate in the cell differentiation of normal and n‐hexadecane‐induced hyperplastic epidermis of the guinea pigs. The epidermal cells were separated into three layers; high (HDCL), middle (MDCL), and low (LDCL) density cell layer, respectively, by Percoll gradient centrifugation. In epidermal homogenate, the activity of DNA polymerase β was higher than that of DNA polymerase α . DNA polymerase α activity was higher in the HDCL than in the other layers; however, DNA polymerase β was higher in the LDCL than in the other layers. In hyperplastic epidermis, distribution of DNA polymerase α activity was similar to the normal pattern, but DNA polymerase β was lower in the LDCL than in the other layers. The distribution of DNA polymerase α activity in epidermal nuclei was similar to that of the whole epidermal cell pattern; however, DNA polymerase β activity differed from the enzyme distribution of whole epidermal cell homogenate. Its activity was higher in the HDCL than in the other layers. In hyperplastic epidermis, both nuclear DNA polymerase α and β activities were similar to the distribution patterns of polymerase activities in hyperplastic epidermal cells. From these results, it is concluded that the distribution pattern of DNA polymerases in n‐hexadecane induced‐hyperplastic epidermis is not a simple augmentation of the distribution pattern in normal epidermis.