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A Simplified Disc Technique for Quantitating Mouse DNA, RNA and Protein Synthesis: Circadian Rhythms
Author(s) -
Yoshino Kazuhiro,
Oba Kenkichi,
Maibach Howard I.
Publication year - 1986
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1986.tb02909.x
Subject(s) - rna , circadian rhythm , hairless , dna synthesis , dna , protein biosynthesis , microbiology and biotechnology , biology , uridine , biochemistry , chemistry , gene , endocrinology
1) A modified disc technique has been developed for quantitating DNA, RNA and protein synthesis in epidermal hairless mouse skin and utilized in examining circadian rhythms. 2) The modified epidermal assay for DNA synthesis, utilizing dissolution of epidermis before scintillation counting, yields three times the specific activity than does the original disc technique. The modified disc technique also gives satisfactory results for RNA and protein synthesis. 3) The incorporation of 3H‐thymidine (3H‐TdR) into DNA was highest from 23:00 to 03:00. RNA synthesis, as measured by 3H‐uridine (3H‐UR) incorporation, and protein synthesis, as measured by 3H‐leucine (3H‐Leu) and 14C‐histidine (14C‐His) incorporation, did not show any obvious circadian rhythm.