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CONTACT SENSITIVITY IN RATS INDUCED BY TOLYLENE DIISOCYANATE (TDI)
Author(s) -
Tanaka KenIchi,
Nagaya Yoshiko,
Marui Shogo,
Okamoto Yutaka,
Hanada Satoshi
Publication year - 1985
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1985.tb02879.x
Subject(s) - swelling , sensitization , medicine , pharmacology , chemistry , immunology , pathology
A model of contact sensitivity (CS) based upon ear swelling has been developed in rats, which are regarded as low responders to common contact sensitizers. For sensitization, a 1% solution of tolylene diisocyanate (TDI) in ethyl acetate was applied to male F344 rats in three different ways; the solution was applied to the shaved backs (1st group), to the unshaved tails (2nd group), and to the tails and then, seven days later, to the backs (3rd group). All the rats were ear‐challenged seven days after the above treatments. The most remarkable ear swelling was observed in the 3rd group, the rats sensitized by the double application method. Delayed onset of the ear response together with histology of the swollen ear showed that the response was characteristic of delayed‐type hypersensitivity. This was confirmed by the successful achievement of CS transfer with lymphocytes from sensitized rats. When picryl chloride was used as a sensitizer instead of TDI, no comparable ear swelling was induced irrespective of the sensitization method. It was also found that topical application of a corticosteroid cream dramatically reduced the ear swelling induced by TDI. These results suggest not only that an experimental model for CS can be developed in rats using TDI, but also that CS induced by TDI can be used as a model for evaluation of anti‐CS drugs.