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EPIDERMAL CELLS BEARING BOTH I‐A AND I‐E SUBREGION ANTIGENS CAN INDUCE HEN EGG WHITE LYSOZYME‐SPECIFIC T LYMPHOCYTE PROLIFERATION *
Author(s) -
Katoh Kiyoshi,
Matsunaga Keiichiro,
Ishii Norihisa,
Okubo Takao,
Okuda Kenji
Publication year - 1985
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1985.tb01534.x
Subject(s) - antigen , antigen presenting cell , biology , antigen presentation , t lymphocyte , immunology , ovalbumin , lymphocyte , immune system , cytotoxic t cell , mixed lymphocyte reaction , major histocompatibility complex , t cell , microbiology and biotechnology , in vitro , biochemistry
Antigen‐primed T lymphocytes require accessory cells to initiate a proliferative response. These cells have been designated antigen presenting cells. In the present investigation, we found that immune associated antigen (Ia) bearing, mouse epidermal cells possess antigen presenting activity. These epidermal cells also display substantial levels of mixed lymphocyte reaction (MLR) activity. However, this MLR of itself did not suppress nor enhance antigen presenting activity. Genetic mapping studies demonstrated that compatibility at the I‐A and I‐E subregions of the MHC is important for effective presentation of either lysozyme or ovalbumin. In vitro inhibition and blocking studies using alloantisera confirmed that I‐A and I‐E gene products of these epidermal cells may play an important role in the interaction of primed T lymphocytes and antigen‐pulsed epidermal cells. In addition, epidermal cells bearing Fc and C3 receptors on the cell surfaces were more efficient at inducing the lymphocyte proliferation than were epidermal cells depleted of these cells. These results suggest that Ia positive epidermal cells may be involved in the initiation of delayed type hypersensitivity reactions through antigen recognition followed by antigen presentation and T lymphocyte proliferation.