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COLLAGEN POLYMORPHISM: TYPE V COLLAGEN IN NEUROFIBROMA
Author(s) -
Shinkai Hiroshi
Publication year - 1984
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1984.tb01441.x
Subject(s) - hydroxylysine , chemistry , collagenase , chromatography , type i collagen , polyacrylamide gel electrophoresis , biochemistry , gel electrophoresis , electrophoresis , microbiology and biotechnology , lysine , biology , enzyme , amino acid , endocrinology
Three types of collagen were found in neurofibroma tumor tissue from a patient with neurofibromatosis (Von Recklinghausen's disease). Two of them were determined to be type I and type III collagen by CM‐cellulose chromatography and SDS‐polyacrylamide gel electrophoresis. The third matched a membrane type collagen from amnionic and chorionic membrane of human placenta in its elution profile during CM‐cellulose chromatography and its electrophoretic patterns on SDS‐polyacrylamide gel. This collagen was precipitated with 3.6‐4.0 M NaCl at neutral pH after limited pepsin digestion. It was composed of two components with molecular weights of approximately 110K and 125K daltons. These collagenous constituents had a high content of hydroxylysine linked disaccharide units (glucosylgalactose) and were resistant to reduction. They were digested by bacterial collagenase (EC 3.4.24.3) but resisted tadpole collagenase (EC 3.4.24.7).