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ABNORMAL DNA HISTOGRAMS OF STROMA CELLS IN PATIENTS WITH TUBEROUS SCLEROSIS
Author(s) -
Ishibashi Yasumasa,
Inoue Yukiko,
Takehara Kazuhiko,
Kukita Atsushi,
Murakami Yasufumi,
Hanaoka Fumio,
Yamada MasaAtsu
Publication year - 1984
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1984.tb01432.x
Subject(s) - tuberous sclerosis , stroma , lesion , pathology , dna , biology , medicine , genetics , immunohistochemistry
Explant cultures were attempted from the so‐called adenoma sebaceum in the face and a plaque lesion on the neck observed in three genetically related cases (three males twelve, fifteen, and 42 years old) with Pringle's disease, tuberous sclerosis. Then the DNA content of outgrown non‐epithelial cells (NEC), or stroma cells, derived from the respective tissues (10,000 nuclei and 15,000 cells) were investigated by a flow cytofluorometer. 1) DNA histograms per cell showed no remarkable differences between patients or lesions. On the other hand, 2) DNA histograms per nucleus did show certain differences. Among similar lesions, higher degrees of involvement showed more remarkable differences. 3) The NEC from the mulberry‐shaped tumor (adenoma sebaceum) showed obviously reduced peaks of 2C and 4C, and an increased width and increase in cells with their DNA content corresponding to the S‐phase. In addition, 4) there were no findings suggesting the presence of cells with excessive DNA content over 4C.