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A STUDY OF THE INTERACTION BETWEEN CHLORPROMAZINE AND RIBOFLAVIN BINDING PROTEIN
Author(s) -
Shiga Akiko,
Matsumoto Syuichi,
Ueda Hiroshi,
Shiga Kiyoshi,
Nishina Yasuzo,
Watari Hiroshi,
Horiike Kihachiro
Publication year - 1983
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1983.tb01166.x
Subject(s) - riboflavin , flavin group , dissociation constant , chlorpromazine , chemistry , dissociation (chemistry) , flavin adenine dinucleotide , cofactor , stereochemistry , biochemistry , enzyme , pharmacology , organic chemistry , biology , receptor
The interaction between chlorpromazine (CPZ) and riboflavin binding protein (RBP) was studied by the spectrophotometric and spectrofluorometric methods. CPZ combined with RBP in a 1:1 molar ratio and the dissociation constant was determined to be 3.8 × 10 –5 M at pH 7, 25°C. Flavins such as riboflavin (Rf) and lumiflavin were released from the complexes with RBP by the addition of CPZ. The mechanism of flavin‐release was mainly competitive binding between CPZ and a isoalloxazine ring of flavins to RBP. The mechanism of some biological phenomena induced by CPZ was discussed based on the flavin‐CPZ interaction.