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DEMONSTRATION OF IMMUNOGLOBULIN G IN NORMAL HUMAN EPIDERMIS BY LIGHT AND ELECTRON MICROSCOPY USING PEROXIDASE LABELED ANTIBODY
Author(s) -
Yamada Mariko
Publication year - 1983
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1983.tb01148.x
Subject(s) - epidermis (zoology) , immunohistochemistry , antibody , peroxidase , staining , pathology , electron microscope , immunoglobulin g , chemistry , human skin , biology , microbiology and biotechnology , enzyme , immunology , anatomy , medicine , biochemistry , optics , physics , genetics
Distribution of immunoglobulins in normal human epidermis was revealed at both the light and electron microscopic levels using peroxidase labeled antibodies. Biopsy specimens were obtained from various body locations of 40 healthy persons from 2 to 82 years old under local anesthesia. There were three types of IgG staining patterns in normal human epidermis. Intraepidermal IgG was found in about 80% of the skin samples. IgG was localized in the cytosol of squamous cells by electron microscopy. There were no morphological differences between the IgG positive and IgG negative cells. There seemed to be a correlation between IgG staining and the IgG serum level. The results of the present study suggest that with highly sensitive immunohistochemical techniques, IgG can be detected not only in diseased skin but also in normal skin.