Premium
IMMUNOLOGICAL STUDIES OF AN EXTRACELLULAR KERATINASE
Author(s) -
Takiuchi Iwao,
Higuchi Dousei,
Sei Yoshihiro,
Koga Miho
Publication year - 1983
Publication title -
the journal of dermatology
Language(s) - English
Resource type - Journals
SCImago Journal Rank - 0.9
H-Index - 65
eISSN - 1346-8138
pISSN - 0385-2407
DOI - 10.1111/j.1346-8138.1983.tb01146.x
Subject(s) - keratinase , sodium dodecyl sulfate , size exclusion chromatography , chemistry , gel electrophoresis , extracellular , enzyme , chromatography , electrophoresis , biochemistry , microbiology and biotechnology , biology
The extracellular keratinase from M. canis was purified using ion exchange chromatography and gel filtration. The purified enzyme showed a single protein band on sodium dodecyl sulfate‐polyacrilamide gel electrophoresis. A molecular weight of approximately 45,000 was determined by SDS‐electrophoresis. Anti‐body directed against the purified enzyme from M. canis was obtained from New Zealand White rabbits, and the immunological identity of the enzyme from M. canis , as well as those from M. gypseum, T. mentagrophytes and T. rubrum , were demonstrated by immuno‐double‐diffusion experiments.